目的 采用析因设计法探讨LASIK术角膜基质床厚度的主要影响因素间的关联。
方法 选取接受LASIK术病人30例60眼，以术前眼压、屈光度、术后角膜基质床厚度为研究因素进行析因设计试验。
结果 术前屈光度间、术前眼压与术前屈光度之间差异无统计学意义（P＞0.05）；术前眼压间（P＜0.05）、术前眼压与术后角膜基质床厚度之间（P＜0.01）及术前屈光度与术后角膜基质床厚度之间（P＜0.01）差异有统计学意义。术前眼压、屈光度、术后角膜基质床厚度间存在交互影响（P＜0.01）。
结论 析因设计法可用于眼科专业中多因素多水平的研究；术前眼压、屈光度、术后角膜基质床厚度是影响LASIK术成功与否的重要因素，在术前眼压低于21mmHg、术前屈光度中度、术后角膜基质床厚度保留300μm以上较安全。
Objective To apply factorial design to investigate the main influence factors of corneal stroma bed thickness after laser in situ keratomileusis (LASIK) and their relevance.
Methods 60 eyes of 30 Cases were randomly selected from patients treated with LASIK. Do factorial design experiments with the main study factors such as preoperative intraocular pressure, degrees of refraction and thickness of postoperative corneal stroma bed . Results The difference between Preoperative dioptres and the difference between Preoperative dioptre and preoperative intraocular pressure are meaningless (P >0.05), there are statistical differences in the others. There are interaction among preoperative intraocular pressure, preoperative dioptre and thickness of postoperative corneal stroma bed (P＜0.05 or P＜0.01).
Conclusion The three factors can influence the surgical results.It will be more safely if preoperative intraocular pressure under 21mmHg,moderate preoperative dioptre and thickness of postoperative corneal stroma bed above 300um.